A hyperspectral microscope is an innovative microscope that integrates conventional microscopy with spectral imaging technology, enabling the simultaneous acquisition of images across multiple wavelengths of light. It provides both spatial information and spectral information of the sample at the microscopic level, capturing spectral data at every pixel of the image.
It enables the acquisition of high-resolution spectral information from samples across different wavelength ranges while simultaneously capturing the morphological structure of biological samples.
This integration of imaging and spectroscopy achieves a seamless fusion of mages and spectra, providing more precise spectral analysis. This groundbreaking advancement in technology integrates the fields of maging and spectroscopy through hyperspectral imaging, allowing simultaneous acquisition of spatial and spectral information.
Integrated Spectra and Images: Seamlessly compatible with machine vision images and multi-dimensional spectral data fusion analysis.
High Precision: Nanometer-level spectral resolution with wavelength coverage spanning UV, visible, and infrared regions.
High Efficiency: High-speed detection and rapid data processing.
In-Situ Capability: No need to share or move the measured object.
NonDestructive: Requires only necessary illumination without any physical contact or damage to the sample
Hyperspectral microscopes can be applied in a wide range of biological research fields, particularly in histopathology, cell biology, and biomaterials. They offer high-resolution fluorescence imaging combined with position-specific spectral analysis, enabling detailed investigations at the cellular and nanostructural levels.
Histological morphology image and corresponding spectral curves for each part
Cytological morphology image and corresponding hyperspectral curves
Spectral curves corresponding to each part of the cell
Spectral curves corresponding to each part of the nanomaterials
Hyperspectral ultra microscopic system~ 3D image construction
High-resolution fluorescent
High-resolution fluorescent
The use of fluorescent techniques (Fluorescent Application) in medical diagnostics becomes even more powerful when integrated with hyperspectral microscopy systems. Hyperspectral microscopes are highly capable tools for analyzing cells at microscopic and subcellular levels with remarkable accuracy and resolution. They play a crucial role in fields such as cancer diagnostics and infectious disease detection by providing precise and localized results.
Benefits of This Application
Simultaneous Detection of Multiple Fluorophores (Multiplexing)
Traditional microscopes typically cannot distinguish fluorophores with similar emission spectra (e.g., green-yellow). In contrast, hyperspectral microscopes can separate these signals into detailed spectra, allowing users to identify distinct sources even with overlapping wavelengths.
Higher Accuracy with Autofluorescence Suppression
Biological samples often exhibit autofluorescence (natural fluorescence from cells), which can interfere with detection. Hyperspectral systems can distinguish between target signals and background autofluorescence more effectively than conventional systems.
Spectral Imaging Within Cells
This technique allows researchers not only to see the location of fluorescence but also to determine what chemical or protein is present at each location, enabling in-depth molecular analysis.
Non-destructive Analysis
Enables live-cell imaging by using fluorescence to monitor cellular behavior and biochemical events in real time, without damaging the sample.